Journal: Oncotarget

Article Title: Lumbar puncture-administered resveratrol inhibits STAT3 activation, enhancing autophagy and apoptosis in orthotopic rat glioblastomas

doi: 10.18632/oncotarget.12414

Figure Lengend Snippet: A. Illustration of less aggressive growth (Border) and distinct cell death (Inside) caused by lumbar puncture (LP) resveratrol through HE staining. B. TUNEL labeling and Cyclin D1 immunohistochemical staining performed on the tumors treated by lumbar punctured 0.3% DMSO (C) and resveratrol (Res). C, D. The incidences of TUNEL-positive and Cyclin D1-positive cells in the two experimental groups.

Article Snippet: In parallel, immunohistochemical staining was performed adjacent sections using a mouse anti-Cyclin D1 antibody (1:80; DAKO, Glostrup, Denmark) to evaluate tumor proliferation [ ].

Techniques: Staining, TUNEL Assay, Labeling, Immunohistochemical staining

Journal: Oncology reports

Article Title: Impact of Mucin1 knockdown on the phenotypic characteristics of the human hepatocellular carcinoma cell line SMMC-7721.

doi: 10.3892/or.2014.3136

Figure Lengend Snippet: Figure 3. Knockdown of MUC1 expression alters the β‑catenin signaling pathway by blocking β‑catenin translocation to the nucleus. (A) Cell lysates from NC and MR1-D4 clones were subjected to immunoprecipitation (IP) with anti-MUC1-CT antibody or normal IgG and then immunoblotted (IB) with anti‑β‑catenin antibody. Whole cell lysate (WCL) was not subjected to immunoprecipitation. (B) Cytoplasmic extracts from SMMC-7721, NC, MR1-C6 and MR1-D4 cells were analyzed by western blotting for the expression of β‑catenin. Cytoplasmic IκBα was used as a protein loading control. (C) Nuclear extracts from SMMC‑7721, NC, MR1-C6 and MR1-D4 cells were detected by western blotting to assess the levels of nuclear β‑catenin. Lamin B1 served as the nuclear loading control. (D) NC, MR1-C6 and MR1-D4 cells were transiently transfected with TOPflash and FOPflash plasmids. Relative luciferase activity was cal culated as the ratio of TOPflash/FOPflash luciferase activity, and each value was normalized to the luciferase activity of the internal control pRL-TK reporter plasmid. (E) mRNA levels of cyclin D1 and c-Myc in NC, MR1-C6 and MR1-D4 cells were detected by qRT-PCR and normalized to β‑actin. Bars represent the relative mRNA level when compared to the NC cells. (F) Cell lysates were analyzed by western blotting for the expression of cyclin D1 and c-Myc. β‑actin was used as a loading control. Data are expressed as the means ± SDs of 3 independent experiments. *P<0.05 compared with NC.

Article Snippet: The primary antibodies used were antibodies against MUC1 (GP1.4) (1:2,000; NeoMarkers), c-Myc (1:1,000), cyclin D1 (1:1,000), β-actin (1:2,000), IκBα (1:2,000) and Lamin B1 (1:2,000; all from Epitomics, Burlingame, CA, USA), β-catenin (1:1,000; BD Biosciences), E-cadherin (Proteintech), caspase-3 (Santa Cruz Biotechnology).

Techniques: Knockdown, Expressing, Blocking Assay, Translocation Assay, Clone Assay, Immunoprecipitation, Western Blot, Control, Transfection, Luciferase, Activity Assay, Plasmid Preparation, Quantitative RT-PCR